Immunofluorescent staining using EPO antibody (10C-CR1196M2)
EPO antibody
Immunofluorescent staining using EPO antibody (10C-CR1196M2)
Western blot analysis using EPO antibody (10C-CR1196M2)
Mouse kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-EPO (1:500 dilution). Arrow indicates glycosylated EPO (~34 kDa).
ELISA assay using EPO antibody (10C-CR1196M2)
EPO concentration was determined to be in the range of 2-200 ng/mL. Antibody used at 5 ug/mL to coat plate. Optimal working dilutions must be determined by the end user.
Immunofluorescent staining using EPO antibody (10C-CR1196M2)
Specifications
Host
Mouse
Clone
813600
Isotype
IgG
Method of Purification
EPO antibody was purified by affinity chromatography.
Form & Buffer
20mM Na3PO4, with 300mM NaCl, and 0.02% C9H9O2SHgNa.
Concentration
Batch dependent - please inquire should you have specific requirements
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.
General Information
Biological Significance
Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.