Estrogen Sulfotransferase protein (His tag) (80R-1125)
Purified recombinant Human Estrogen Sulfotransferase protein
|Synonyms||Estrogen-preferring EST-1 protein, estrone sulfotransferase protein, EST-1 protein, member 1 EST1 protein, STE protein, Sulfotransferase family 1E protein, STE protein, EST protein, SULT1E1 protein, Sulfotransferase estrogen preferring., estrogen-preferring protein|
Coomassie Blue stained SDS-PAGE of Estrogen Sulfotransferase protein (His tag) (80R-1125)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-294 amino acids: MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN PGSLPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEILEHHHH HH|
|Grade & Purity||> 95% pure|
|Molecular Weight||36.1 kDa (302aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. Estrogen sulfotransferase may control the level of the estrogen receptor by sulfurylating free estradiol. Recombinant SULT1E1, fused to His-tag at C-terminus, was expressed in E. coli and was purified by conventional chromatography.|
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