EXO1 antibody (70R-50682)

Purified Polyclonal EXO1 antibody

Synonyms Polyclonal EXO1 antibody, Anti-EXO1 antibody, EXOI antibody, HEX1 antibody, Exonuclease 1 antibody, hExo1 antibody, Exonuclease I antibody, hExoI antibody
Specificity Recognizes endogenous levels of EXO1 protein
Cross Reactivity Mouse
Applications ChIP, ICC, IF, WB
Immunogen EXO1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EXO1 as the immunogen

Images

Western blot analysis using EXO1 antibody (70R-50682)

Western blot analysis of EXO1 expression in Raw264.7 (A); A431 (B) whole cell lysates.

Specifications

Host Rabbit
Method of Purification EXO1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.

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Images

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Western blot analysis using EXO1 antibody (70R-50682)



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Western blot analysis of EXO1 expression in Raw264.7 (A); A431 (B) whole cell lysates.
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Immunofluorescent analysis using EXO1 antibody (70R-50682)



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Immunofluorescent analysis of EXO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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