EXO1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EXO1 as the immunogen
Western blot analysis using EXO1 antibody (70R-50682)
Western blot analysis of EXO1 expression in Raw264.7 (A); A431 (B) whole cell lysates.
EXO1 antibody
Western blot analysis using EXO1 antibody (70R-50682)
Western blot analysis of EXO1 expression in Raw264.7 (A); A431 (B) whole cell lysates.
Immunofluorescent analysis using EXO1 antibody (70R-50682)
Immunofluorescent analysis of EXO1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
EXO1 antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.