Fas Receptor protein (30R-AF031)

Purified recombinant Human Fas Receptor protein

Synonyms sFasR protein, soluble Fas receptor protein, Apo I protein, CD95 protein, Fas Antigen protein, TNFRSF6 protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Residues Region of Fas Receptor protein corresponding to amino acids MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS.
Expression System E.coli
Grade & Purity > 98% pure
Molecular Weight 17.6 kDa
Form & Buffer Supplied lyophilized with no additives. Reconstitute in water to a concentration of 0.1-1.0 mg/ml.

Usage & Assay Information

Bioactivity The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 ug/ml in the presence of 2ng/ml of hFasL.

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels Endotoxin level is less than 0.1 ng per ug (1 EU/ug).

General Information

Biological Significance Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.

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Availability: In stock

Price: €211.69
Size: 20 ug
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