FAST antibody (70R-12677)

Affinity purified Rabbit polyclonal FAST antibody

Synonyms Polyclonal FAST antibody, Anti-FAST antibody, FASTK antibody, fas-activated serine/threonine kinase antibody, FAST antibody, FAST kinase antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, IHC-P, WB
Immunogen FAST antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 346 and 549 of FAST

Images

Western blot analysis using FAST antibody (70R-12677)

Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and FAST antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification FAST antibody was purified by Protein A chromatography
Molecular Weight 61 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 6.56 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using FAST antibody (70R-12677)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 7.5 % SDS PAGE gel and FAST antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using FAST antibody (70R-12677)</font></div>     | Immunohistochemical staining of paraffin-embedded SCC15 xenograft using FAST antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using FAST antibody (70R-12677)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: MOLT4; B: Raji) using a 12 % SDS PAGE gel and FAST antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Immunofluorescent staining using FAST antibody (70R-12677)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using FAST antibody at 1:200 dilution.

Availability: In stock

Price: €323.11
Size: 100 ul
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