FBL protein (His tag) (80R-1483)
Purified recombinant Human FBL protein
|Synonyms||RNU3IP1 protein, rRNA 2'-O-methyltransferase fibrillarin, FIB protein, FLRN protein|
Coomassie Blue stained SDS-PAGE of FBL protein (His tag) (80R-1483)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||83-321 amino acids: MGSSHHHHHH SSGLVPRGSH RSMGKNVMVE PHRHEGVFIC RGKEDALVTK NLVPGESVYG EKRVSISEGD DKIEYRAWNP FRSKLAAAIL GGVDQIHIKP GAKVLYLGAA SGTTVSHVSD IVGPDGLVYA VEFSHRSGRD LINLAKKRTN IIPVIEDARH PHKYRMLIAM VDVIFADVAQ PDQTRIVALN AHTFLRNGGH FVISIKANCI DSTASAEAVF ASEVKKMQQE NMKPQEQLTL EPYERDHAVV VGVYRPPPKV KN|
|Grade & Purity||> 85% pure|
|Molecular Weight||28.9 kDa (262aa) , confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 1mM DTT, 0.2M NaCl and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||FBL is a key small nucleolar protein in eukaryotes, which has an important role in pre-rRNA processing during ribosomal biogenesis. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. This protein is a component of several ribonucleoproteins including a nucleolar small nuclear ribonucleoprotein (SnRNP) and one of the two classes of small nucleolar ribonucleoproteins (snoRNPs). Recombinant human FBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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