FEN1 protein (30R-1442)

Purified recombinant Human FEN1 protein

Synonyms Flap structure specific endonuclease 1 protein, Flap endonuclease 1 protein, Maturation factor 1 protein, MF1 protein, hFEN1 protein, RAD2 protein, FEN 1, FEN-1, Flap structure-specific endonuclease 1 DNase IV protein, FEN 1 protein, FEN-1 protein, FEN-1 protein, , FEN1
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of FEN1 protein (30R-1442)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-380 amino acids: MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 42.5 kDa (380aa), confirmed by MALDI-TOF.
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of FEN1 protein (30R-1442) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 100 ug
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