Ferredoxin 1 protein (T7 tag) (80R-1177)
Purified recombinant Human Ferredoxin 1 protein (T7 tag)
Overview
Overview
| Synonyms | Hepatoredoxin protein, Adrenodoxin protein, Ferredoxin 1, Ferredoxin 1, Adrenal ferredoxin protein, ADX protein, Ferredoxin -1 protein, Ferredoxin 1 protein, , FDX1 protein, Ferredoxin -1, Adrenodoxin protein, LOH11CR1D protein, FDX protein, mitochondrial protein, Ferredoxin 1 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of Ferredoxin 1 protein (T7 tag) (80R-1177)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 61-184 amino acids: MASMTGGQQM GRGSMSSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 15.0 kDa (139aa), confirmed by MALDI-TOF. |
| Tag/Conjugate | T7 tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | < 1.0 EU per ug of protein (determined by LAL method) |
General Information
| Biological Significance | Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
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