Ferritin antibody (20R-FR001)
Rabbit polyclonal Ferritin antibody
Overview
Overview
| Synonyms | Polyclonal Ferritin antibody, Anti-Ferritin antibody, Ferritin L subunit antibody, Ferritin light chain like antibody, FTH antibody, Ferritin heavy chain like antibody, Ferritin H subunit antibody, Ferritin light polypeptide antibody, Ferritin heavy polypeptide antibody, Ferritin heavy polypeptide 1 antibody |
|---|---|
| Specificity | Human ferritin |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | Ferritin antibody was raised in rabbit using Ferritin [human Spleen] as the immunogen. |
Images
Western blot analysis using Ferritin antibody (20R-FR001)
Western Blot of Ferritin antibody. Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Ferritin primary antibody at 1:1,000 overnight at 4 deg C. Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 20 kDa, 20 kDa for Ferritin..
Specifications
| Host | Rabbit |
|---|---|
| Method of Purification | Ferritin antibody was purified by ion exchange chromatography followed by dialysis. |
| Form & Buffer | 0.02M K3PO4, pH 7.2, with 0.15M NaCl and 0.01% NaN3. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:5,000-1:20,000, IHC: 1:200-1:1,000, WB: 1:5,000-1:2,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms, including bacteria, algae and higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. |
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