FGF basic protein (30R-AF015)

Purified recombinant Human FGF basic protein

Synonyms FGF-2 protein, HBGH-2 protein, HBGF-2 protein, FGF-b protein, Fibroblast Growth Factor Basic protein, Prostatropin protein
Species Human
Protein Type Recombinant
Applications User optimized
Assay Information Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.

Specifications

Residues Fibroblast Growth Factor protein containing 155 amino acids.
Expression System E.coli
Grade & Purity > 98% pure
Method of Purification FGF protein (Basic)
Molecular Weight 17,353 Da
Form & Buffer Sterile Filtered Lyophilized from a concentrated solution containing PBS pH 7.4. Reconstitute in distilled water to at least 100 ug/ml.

Usage & Assay Information

Bioactivity The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

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