FGF basic protein (30R-AF015)
Purified recombinant Human FGF basic protein
Overview
Overview
| Synonyms | FGF-2 protein, HBGH-2 protein, HBGF-2 protein, FGF-b protein, Fibroblast Growth Factor Basic protein, Prostatropin protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
| Assay Information | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard. |
Specifications
| Residues | Fibroblast Growth Factor protein containing 155 amino acids. |
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| Expression System | E.coli |
| Grade & Purity | > 98% pure |
| Method of Purification | FGF protein (Basic) |
| Molecular Weight | 17,353 Da |
| Form & Buffer | Sterile Filtered Lyophilized from a concentrated solution containing PBS pH 7.4. Reconstitute in distilled water to at least 100 ug/ml. |
Usage & Assay Information
| Bioactivity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
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