FGF21 protein (His tag) (80R-1109)

Purified recombinant Human FGF21 protein

Synonyms FGF 21 protein, Fibroblast growth factor 21 protein, FGF21 protein, FGF-21 protein, Fibroblast growth factor 21 protein, FGF-21, Fibroblast Growth Factor-21, FGF 21, FGF21
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Cell Line: NIH-3T3 (Mus mosculus/ embryo) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay Medium: serum free RPMI 1640 4. Cell density: 20,000 cells/well (96 well plate, final volume 100ul) 5. Serum Free Starvation: 24hr with RPMI1640 6. Incubation Time: 24 hr (after sample treatment) 7. Concentration Range: 1 pg/ml - 10 ug/ml (10-fold dilution) > 8. Detection method: BrdU assay

Images

Coomassie Blue stained SDS-PAGE of FGF21 protein (His tag) (80R-1109)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 29-209 amino acids: MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 21.6 kDa (202 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity The ED50 for this effect is < 12 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 83,000 units/ml).

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance FGF-21 is a unique member of the FGF superfamily that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. Recombinant human FGF-21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of FGF21 protein (His tag) (80R-1109) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €169.35
Size: 100 ug
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