FHL5 antibody (70R-13057)

Affinity purified Rabbit polyclonal FHL5 antibody

Synonyms Polyclonal FHL5 antibody, Anti-FHL5 antibody, FLJ33049 antibody, dJ393D12.2 antibody, FHL 5 antibody, FHL-5 antibody, FHL5, FHL5 antibody, LIM protein ACT antibody, FHL-5 antibody, activator of cAMP-responsive element modulator (CREM) in testis antibody, activator of CREM in testis antibody, RP3-393D12.2 antibody, FHL 5, FHL-5, ACT antibody, activator of cAMP-responsive element modulator in testis antibody, KIAA0776 antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen FHL5 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 186 of FHL5

Images

Immunofluorescent staining using FHL5 antibody (70R-13057)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5 antibody at 1:500 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification FHL5 antibody was purified by antigen-affinity chromatography
Molecular Weight 33 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The protein encoded by this gene is coordinately expressed with activator of cAMP-responsive element modulator. It is associated with CREM and confers a powerful transcriptional activation function. CREM acts as a transcription factor essential for the differentiation of spermatids into mature spermatozoa. There are multiple polyadenylation sites found in this gene.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using FHL5 antibody (70R-13057)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FHL5 antibody at 1:500 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using FHL5 antibody (70R-13057)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: 293T; B: Raji) using a 12 % SDS PAGE gel and FHL5 antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using FHL5 antibody (70R-13057)</font></div>     | Immunohistochemical staining of paraffin-embedded SW480 xenograft using FHL5 antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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