Fibronectin antibody (70R-FR003)

Affinity purified Rabbit polyclonal Fibronectin antibody

Synonyms Polyclonal Fibronectin antibody, Anti-Fibronectin antibody, Transformation sensitive protein antibody, Migration stimulating factor antibody, Cold insoluble globulin antibody, MSF antibody, LETS antibody, CIG antibody
Specificity Human fibronectin
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, IP, WB
Immunogen Fibronectin antibody was raised in rabbit using fibronectin purified from human plasma as the immunogen.

Specifications

Host Rabbit
Method of Purification Fibronectin antibody was purified by affinity chromatography.
Form & Buffer Sterile filtered liquid in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:5,000-1:10,000, IHC: 1:50-1:200, IP: 1:100, WB: 1:5,000-1:10,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Human fibronectin has a molecular weight of 450,000 daltons when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa should be observed after SDS-PAGE when reducing and denaturing conditions are used.

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Price: €1,114.11
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