Filamin B antibody (70R-12564)

Affinity purified Rabbit polyclonal Filamin B antibody

Synonyms Polyclonal Filamin B antibody, Anti-Filamin B antibody, filamin-3 antibody, SCT antibody, thyroid autoantigen antibody, ABP-280 antibody, LRS1 antibody, FLNB antibody, Larsen syndrome 1 (autosomal dominant) antibody, actin binding protein 278 antibody, ABP-280 homolog antibody, AOI antibody, actin-binding-like protein antibody, beta-filamin antibody, TAP antibody, ABP-278 antibody, DKFZp686A1668 antibody, FH1 antibody, DKFZp686O033 antibody, filamin homolog 1 antibody, FLN1L antibody, filamin-B antibody, TABP antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen Filamin B antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 30 and 269 of Filamin B

Images

Western blot analysis using Filamin B antibody (70R-12564)

Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 5 % SDS PAGE gel and Filamin B antibody at a dilution of 1:1000

Specifications

Host Rabbit
Isotype IgG
Method of Purification Filamin B antibody was purified by Protein A chromatography
Molecular Weight 278 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 10 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using Filamin B antibody (70R-12564)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:NIH-3T3) using a 5 % SDS PAGE gel and Filamin B antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunofluorescent staining using Filamin B antibody (70R-12564)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
  • <div><font face=Calibri size=2>Western blot analysis using Filamin B antibody (70R-12564)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 5 % SDS PAGE gel and Filamin B antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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