FITC antibody (biotin) (60R-FG003bt)
Goat polyclonal FITC antibody (biotin) conjugated
Overview
Overview
| Synonyms | Polyclonal FITC antibody, Anti-FITC antibody, Fluorescein antibody |
|---|---|
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | FITC antibody (biotin) was raised in goat using fluorescein conjugated to goat IgG as the immunogen. |
Images
Western blot analysis using FITC antibody (biotin) (60R-FG003bt)
Western Blot of Biotin Conjugated Fluorescein antibody. Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Fluorescein antibody at 1:1000 for 60 min at RT. Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 70 kDa, 70 kDa for BSA-FITC..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | FITC antibody (biotin) was purified by affinity chromatography. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
|---|
Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
|---|---|
| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
|---|
Reviews
You need to be logged in to write a review. Please login here
Sorry there are currently no reviews for this product