FKBP1a /FKBP12 protein (His tag) (80R-1130)

Purified recombinant Human FKBP1a /FKBP12 protein

Synonyms FK506 binding protein 1a protein, FK506 binding protein 12 protein, FKBP1 protein, FKBP12C protein, FK506 binding protein T cell 12 kD protein, Protein kinase C inhibitor 2., FK506-binding protein 1A protein, 12kDa 12 kDa FKBP protein, PPIase FKBP1A protein, PKC12 protein, FKBP 12 protein, FK506 binding protein 1 protein, Rotamase protein, Immunophilin FKBP12 protein, PKCI2 protein, Peptidyl-prolyl cis-trans isomerase protein, FKBP 1A protein, PPIase protein, FK506 binding protein 1A 12kDa protein, FKBP12 Exip3 protein, FKBP12 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant FKBP1a protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1 DEG C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Images

Coomassie Blue stained SDS-PAGE of FKBP1a /FKBP12 protein (His tag) (80R-1130)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-108 amino acids: MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 14.1 kDa (128aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 100mM NaCl, 1mM DTT, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 60 nM/min/ug, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1,4,5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of FKBP1a /FKBP12 protein (His tag) (80R-1130) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €206.99
Size: 100 ug
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