FLAG Antibody (70R-10649)

Affinity purified Chicken polyclonal FLAG antibody

Synonyms Polyclonal FLAG Antibody, Anti-FLAG Antibody, FLAG octapeptide antibody, DYKDDDDK Epitope Tag, FLAG tag antibody, DYKDDDDK antibody
Applications User optimized
Immunogen a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin

Specifications

Host Chicken
Method of Purification FLAG Antibody was purified by affinity chromatography.
Form & Buffer Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.02% sodium azide added as a preservative.
Concentration 100 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term storage add glycerol, aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles. Protect from light.

General Information

Biological Significance FLAG is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

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Availability: In stock

Price: €302.66
Size: 1 ml
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