Immunofluorescent staining using Flightless I antibody (70R-12974)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Flightless I antibody
Immunofluorescent staining using Flightless I antibody (70R-12974)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Immunofluorescent staining using Flightless I antibody (70R-12974)
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1:200 dilution.
Western blot analysis using Flightless I antibody (70R-12974)
Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000
Western blot analysis using Flightless I antibody (70R-12974)
Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
Flightless I antibody was purified by antigen-affinity chromatography
Molecular Weight
145 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.