Flightless I antibody (70R-12974)

Affinity purified Rabbit polyclonal Flightless I antibody

Synonyms Polyclonal Flightless I antibody, Anti-Flightless I antibody, Fli1 antibody, FLII antibody, MGC39265 antibody, FLI antibody, protein flightless-1 homolog antibody, flightless I homolog (Drosophila) antibody, FLIL antibody
Cross Reactivity Human, Mouse
Applications ICC, IF, WB
Immunogen Flightless I antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I

Images

Immunofluorescent staining using Flightless I antibody (70R-12974)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.

Specifications

Host Rabbit
Isotype IgG
Method of Purification Flightless I antibody was purified by antigen-affinity chromatography
Molecular Weight 145 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.88 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using Flightless I antibody (70R-12974)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
  • <div><font face=Calibri size=2>Immunofluorescent staining using Flightless I antibody (70R-12974)</font></div>     | Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I antibody at 1:200 dilution.
  • <div><font face=Calibri size=2>Western blot analysis using Flightless I antibody (70R-12974)</font></div>     | Western blot analysis of 30 ug whole cell lysate (A:Raji) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Western blot analysis using Flightless I antibody (70R-12974)</font></div>     | Western blot analysis of 50 ug of whole cell lysate (A: Mouse brain) using a 5 % SDS PAGE gel and Flightless I antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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