FLT3 Ligand protein (30R-2215)
Purified recombinant Human FLT3 Ligand protein
Overview
Overview
| Synonyms | FLT-3, FLT 3, FLT3, FLT-3L protein, FMS-like tyrosine kinase 3 protein, FLT 3 protein, FLT-3 protein, STK-1 ligand protein |
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| Species | Human |
| Protein Type | Recombinant |
| Applications | User optimized |
| Assay Information | This recombinant mouse IL-5 has been tested by sandwich ELISA using the anti-mouse IL-5 antibody pair (TRFK5/TRFK4), and in bioassays using the IL-5 responsive human cell line TF-1. The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x107 Units/mg. |
Specifications
| Expression System | Insect cells |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | FLT3 Ligand protein was purified by affinity chromatography. |
| Molecular Weight | 19-23 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM NaH2PO4, 0.15M NaCl, pH7.2 with 1% BSA, 0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The ED50 of this protein, as measured by IL-6induction in M1 cells, is 5-15 ng/ml in the presence of 1 ng/mlMouse LIF Recombinant Protein. This corresponds to a specific activity of 2 x 105 - 6.7 x 10^4 Units/mg. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
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| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Flt3 Ligand, also known as Flk2 Ligand, is a growth factor that regulates proliferation of early hematopoietic cells. Flt3 Ligand binds to cells expressing the tyrosine kinase receptor Flt3. By itself, Flt3 Ligand does not stimulate proliferation of early hematopoietic cells. Instead, it synergizes with other colony-stimulating factors and interleukins to induce growth and differentiation. Unlike CSFs, however, Flt3 Ligand exerts no activity on mast cells. Multiple isoforms of Flt3 Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein. The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Human Flt3 Ligand shares 71% and 65% amino acid sequence identity with mouse and rat Flt3 Ligand, respectively. Human and mouse Flt3 Ligand show cross-species activity. Recombinant human Flt3 Ligand is a soluble 17.6 kDa protein consisting of 155 amino acid residues. |
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