FOXO1 antibody (70R-49750)

Purified Polyclonal FOXO1 antibody

Synonyms Polyclonal FOXO1 antibody, Anti-FOXO1 antibody, FKHR antibody, FOXO1A antibody, Forkhead box protein O1 antibody, Forkhead box protein O1A antibody, Forkhead in rhabdomyosarcoma antibody
Specificity Recognizes endogenous levels of FOXO1 protein
Cross Reactivity Bovine, Monkey, Pig
Applications ChIP, ICC, IF, IHC, WB
Immunogen FOXO1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FOXO1 as the immunogen

Images

Western blot analysis using FOXO1 antibody (70R-49750)

Western blot analysis of FOXO1 expression in HeLa (A); MCF7 (B); Jurkat (C) whole cell lysates.

Specifications

Host Rabbit
Method of Purification FOXO1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance FOXO1 is a transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress.

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Images

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Western blot analysis using FOXO1 antibody (70R-49750)



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Western blot analysis of FOXO1 expression in HeLa (A); MCF7 (B); Jurkat (C) whole cell lysates.
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Immunohistochemical analysis using FOXO1 antibody (70R-49750)



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Immunohistochemical analysis of FOXO1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using FOXO1 antibody (70R-49750)



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Immunofluorescent analysis of FOXO1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Price: €197.96
Size: 100 ul
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