G3BP1 protein (His tag) (80R-1601)

Purified recombinant Human G3BP1 protein

Synonyms GBP1-3 protein, HDH VIII protein, G3BP1 protein, G3BP1, ATP dependent DNA helicase VIII protein, RasGAP associated endoribonuclease G3BP, GAP SH3 domain binding protein 1 protein, GBP1 3, GBP1 3 protein, Ras GTPase activating protein SH3 domain binding protein protein, G3BP protein, GAP binding protein protein, Ras GTPase activating protein binding protein 1 protein, MGC111040 protein, GBP1-3
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of G3BP1 protein (His tag) (80R-1601)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-466 amino acids: MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD QRVREQRINI PPQRGPRPIR EAGEQGDIEP RRMVRHPDSH QLFIGNLPHE VDKSELKDFF QSYGNVVELR INSGGKLPNF GFVVFDDSEP VQKVLSNRPI MFRGEVRLNV EEKKTRAARE GDRRDNRLRG PGGPRGGLGG GMRGPPRGGM VQKPGFGVGR GLAPRQVEHH HHHH
Expression System E.coli
Grade & Purity > 85% pure
Molecular Weight 53.2 kDa (474aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 100mM NaCl.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance G3BP1 is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. And it cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. Recombinant human G3BP1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of G3BP1 protein (His tag) (80R-1601) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €319.89
Size: 50 ug
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