G6PD antibody (10R-1155)

Mouse monoclonal G6PD antibody

Synonyms Monoclonal G6PD antibody, Anti-G6PD antibody, Glucose-6-phosphate 1-dehydrogenase G6PD antibody, Glucose 6 phosphate 1 dehydrogenase antibody, GPD 6 antibody, GPD -6, zwf antibody, GPD 6, Zwf1p. antibody, Glucose-6-phosphate 1-dehydrogenase antibody, MET19 antibody, G6PD, G6PD1 antibody, GPD -6 antibody, POS10 antibody, G6pdx antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, WB
Immunogen G6PD antibody was raised in mouse using recombinant human G6PD (35-506aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of G6PD antibody (10R-1155)

Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2F6
Isotype IgG2b kappa
Source Anti-human G6PD antibody, clone AT2F6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human G6PD protein.
Method of Purification G6PD antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000-1:2,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia.

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Images

  • Western Blot analysis of G6PD antibody (10R-1155) | Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €249.33
Size: 100 ul
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