GAI2 antibody (70R-11715)
Rabbit polyclonal GAI2 antibody
Overview
Overview
| Synonyms | Polyclonal GAI2 antibody, anti-GAI2 antibody, GNAI2B antibody, GAI 2 antibody, Guanine nucleotide binding protein G antibody, GAI 2, GAI-2, guanine nucleotide binding protein antibody, GAI2, GAI-2 antibody |
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| Cross Reactivity | Human |
| Applications | IP, WB |
| Immunogen | GAI2 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 229 of human Gai2 as the immunogen. |
| Assay Information | GAI2 Blocking Peptide, catalog no. 33R-10684, is also available for use as a blocking control in assays to test for specificity of this GAI2 antibody |
Images
Western Blot analysis using GAI2 antibody (70R-11715)
Western blot analysis of GAI2 expression in 3T3 cell lysate.
Specifications
| Host | Rabbit |
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| Isotype | IgG |
| Method of Purification | GAI2 antibody was purified by affinity chromatography |
| Form & Buffer | Supplied in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
| Concentration | 500 ug/ml |
Usage & Assay Information
| Usage Recommendations | WB: 0.5-4 ug/ml |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Gai2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gai3 and Gai1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gai2 is expressed widely in tissues and cell lines. Activation of PLC-beta by Gai-coupled receptors is the result of release of Gbetag subunits from Gai. Gai2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase. |
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