Immunofluorescent staining using GALNS antibody (70R-13351)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
GALNS antibody
Immunofluorescent staining using GALNS antibody (70R-13351)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Western blot analysis using GALNS antibody (70R-13351)
Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and GALNS antibody at a dilution of 1:1000
Immunohistochemical staining using GALNS antibody (70R-13351)
Immunohistochemical staining of paraffin-embedded NCI-N87 xenograft using GALNS antibody at a dilution of 1:100
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
GALNS antibody was purified by antigen-affinity chromatography
Molecular Weight
58 kDa
Form & Buffer
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder.