GALNS antibody (70R-13351)

Affinity purified Rabbit polyclonal GALNS antibody

Synonyms Polyclonal GALNS antibody, Anti-GALNS antibody, GALNAC6S antibody, galactose-6-sulfate sulfatase antibody, FLJ98217 antibody, GAS antibody, GALNS antibody, FLJ42844 antibody, FLJ17434 antibody, MPS4A antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB

Images

Immunofluorescent staining using GALNS antibody (70R-13351)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.

Specifications

Host Rabbit
Isotype IgG
Method of Purification GALNS antibody was purified by antigen-affinity chromatography
Molecular Weight 58 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using GALNS antibody (70R-13351)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GALNS antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
  • <div><font face=Calibri size=2>Western blot analysis using GALNS antibody (70R-13351)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and GALNS antibody at a dilution of 1:1000
  • <div><font face=Calibri size=2>Immunohistochemical staining using GALNS antibody (70R-13351)</font></div>     | Immunohistochemical staining of paraffin-embedded NCI-N87 xenograft using GALNS antibody at a dilution of 1:100

Availability: In stock

Price: €323.11
Size: 100 ul
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