Gamma actin 1 antibody (70R-49329)

Purified Polyclonal Gamma actin 1 antibody

Synonyms Polyclonal Gamma actin 1 antibody, Anti-Gamma actin 1 antibody, ACTB antibody, Actin cytoplasmic 1 antibody, Beta-actin antibody, POTEKP antibody, ACTBL3 antibody, FKSG30 antibody, Putative beta-actin-like protein 3 antibody, Kappa-actin antibody, POTE ankyrin domain family member K antibody, ACTG1 antibody, ACTB antibody, ACTG antibody, Actin cytoplasmic 2 antibody, Gamma-actin antibody
Specificity Recognizes endogenous levels of Gamma-actin-1 protein
Cross Reactivity Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Sheep
Applications IHC, WB
Immunogen Gamma actin 1 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-actin-1 as the immunogen

Images

Western blot analysis using Gamma actin 1 antibody (70R-49329)

Western blot analysis of Gamma-actin-1 expression in HeLa (A); Raw264.7 (B); PC12 (C); L929 (D); C6 (E) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Gamma actin 1 antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Gamma actin 1 is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss.

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Images

  • 

Western blot analysis using Gamma actin 1 antibody (70R-49329)



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Western blot analysis of Gamma-actin-1 expression in HeLa (A); Raw264.7 (B); PC12 (C); L929 (D); C6 (E) whole cell lysates.
  • 

Immunohistochemical analysis using Gamma actin 1 antibody (70R-49329)



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Immunohistochemical analysis of Gamma-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Availability: In stock

Price: €197.96
Size: 100 ul
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