Gamma enolase antibody (70R-49706)

Purified Polyclonal Gamma enolase antibody

Synonyms Polyclonal Gamma enolase antibody, Anti-Gamma enolase antibody, Gamma-enolase antibody, 2-phospho-D-glycerate hydro-lyase antibody, Enolase 2 antibody, Neural enolase antibody, Neuron-specific enolase antibody, NSE antibody
Specificity Recognizes endogenous levels of Gamma-enolase protein
Cross Reactivity Mouse, Rat, Chicken, Monkey
Applications FC, ICC, IF, IHC, WB
Immunogen Gamma enolase antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-enolase as the immunogen

Images

Western blot analysis using Gamma enolase antibody (70R-49706)

Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.

Specifications

Host Rabbit
Method of Purification Gamma enolase antibody was purified by immunogen affinity chromatography
Form & Buffer Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide

Usage & Assay Information

Usage Recommendations WB: 1:500 - 1:1000, IHC: 1:100 - 1:200, IF: 1:100 - 1:500, ICC: 1:100 - 1:500, FC: 1:100 - 1:200

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles

General Information

Biological Significance Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

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Images

  • 

Western blot analysis using Gamma enolase antibody (70R-49706)



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Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.
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Immunohistochemical analysis using Gamma enolase antibody (70R-49706)



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Immunohistochemical analysis of Gamma-enolase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis using Gamma enolase antibody (70R-49706)



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Immunofluorescent analysis of Gamma-enolase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Availability: In stock

Price: €197.96
Size: 100 ul
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