Recognizes endogenous levels of Gamma-enolase protein
Cross Reactivity
Mouse, Rat, Chicken, Monkey
Applications
FC, ICC, IF, IHC, WB
Immunogen
Gamma enolase antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-enolase as the immunogen
Western blot analysis using Gamma enolase antibody (70R-49706)
Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.
Gamma enolase antibody
Western blot analysis using Gamma enolase antibody (70R-49706)
Western blot analysis of Gamma-enolase expression in U937 (A); K562 (B); Jurkat (C); NIH3T3 (D); KNRK (E); HepG2 (F); HeLa (G) whole cell lysates.
Immunohistochemical analysis using Gamma enolase antibody (70R-49706)
Immunohistochemical analysis of Gamma-enolase staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis using Gamma enolase antibody (70R-49706)
Immunofluorescent analysis of Gamma-enolase staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Specifications
Host
Rabbit
Method of Purification
Gamma enolase antibody was purified by immunogen affinity chromatography
Form & Buffer
Supplied in liquid form in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3 with 30% glycerol and 0.01% sodium azide
Store at 4 deg C for short term storage. For long term, aliquot and store at -20 deg C. Avoid repeat freeze/thaw cycles
General Information
Biological Significance
Gamma enolase has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.