GAPDH antibody (10R-1178)

Mouse monoclonal GAPDH antibody

Synonyms Monoclonal GAPDH antibody, Anti-GAPDH antibody, wu:fb33a10. antibody, MGC105239 antibody, MGC102546 antibody, MGC102544 antibody, Glyceraldehyde-3-phosphate dehydrogenase 38 kDa BFA-dependent ADP-ribosylation substrate antibody, E 1c.2.1.12 antibody, OTTHUMP00000174431 antibody, BARS-38 antibody, MGC103190 antibody, Glyceraldehyde 3 phosphate dehydrogenase antibody, Aging-associated gene 9 protein antibody, MGC103191 antibody, OCAS antibody, cb609 antibody, OCT1 coactivator in S phase antibody, G3PD antibody, MGC127711 antibody, GAPDH antibody, MGC88685 antibody, KNC-NDS6 antibody, GAPD antibody, p38 component antibody, OTTHUMP00000174432 antibody, G3PDH antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody, 38-KD component antibody
Specificity Human
Cross Reactivity To be determined by end-user.
Applications ELISA, IF, WB
Immunogen GAPDH antibody was raised in mouse using recombinant human GAPDH (1-335aa) purified from E.coli as the immunogen.

Images

Immunofluorescent staining using GAPDH antibody (10R-1178)

Immunofluorescenitrocellulosee of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red (Red).

Specifications

Host Mouse
Clone AT8G4
Isotype IgG2b kappa
Source Anti-human GAPDH antibody, clone AT8G4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GAPDH protein.
Method of Purification GAPDH antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:500-1:10,00, WB: 1:500-1:10,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Multiple roles for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been recently appreciated. GAPDH is found in the particulate fractions, such as the nucleus, the mitochondria, and the small vesicular fractions. GAPDH gene expression is specifically increased during programmed neuronal cell death. When cells are exposed to various stressors, dynamic subcellular re-distribution of GAPDH occurs. GAPDH is also involved in various diseases, especially neurodegenerative disorders and cancers. As a membrane protein, GAPDH functions in endocytosis; in the cytoplasm, it is involved in the translational control of gene expression; in the nucleus, it functions in nuclear tRNA export, in DNA replication, and in DNA repair.

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Images

  • Immunofluorescent staining using GAPDH antibody (10R-1178) | Immunofluorescenitrocellulosee of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human GAPDH antibody (1:500) with Texas Red (Red).
  • Western Blot analysis of GAPDH antibody (10R-1178) | Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human GAPDH. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: €277.55
Size: 100 ul
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