GAPDH antibody (10R-2932)
Mouse monoclonal GAPDH antibody
Overview
Overview
| Synonyms | Monoclonal GAPDH antibody, Anti-GAPDH antibody, GAPDH Loading Control antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody |
|---|---|
| Specificity | Human |
| Cross Reactivity | Mouse, rat, rabbit, chicken, hamster |
| Applications | Dot Blot, ELISA, WB |
| Immunogen | GAPDH antibody was raised in mouse using recombinant GAPDH as the immunogen. |
Images
Western Blot analysis using GAPDH antibody (10R-2932 (1))
1:2000 (0. 5 ug/ml) antibody dilution used in WB of 5 ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5) and hamster (6).
Specifications
| Host | Mouse |
|---|---|
| Clone | GA1R |
| Isotype | IgG1 |
| Method of Purification | GAPDH antibody was purified by Protein A affinity chromatography. |
| Form & Buffer | Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3 |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1:1000-100,000 |
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Storage & Safety
| Storage | Store at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. |
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