GAPDH antibody (10R-2932)

Mouse monoclonal GAPDH antibody

Synonyms Monoclonal GAPDH antibody, Anti-GAPDH antibody, GAPDH Loading Control antibody, Glyceraldehyde-3-phosphate dehydrogenase antibody
Specificity Human
Cross Reactivity Mouse, rat, rabbit, chicken, hamster
Applications Dot Blot, ELISA, WB
Immunogen GAPDH antibody was raised in mouse using recombinant GAPDH as the immunogen.

Images

Western Blot analysis using GAPDH antibody (10R-2932 (1))

1:2000 (0. 5 ug/ml) antibody dilution used in WB of 5 ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5) and hamster (6).

Specifications

Host Mouse
Clone GA1R
Isotype IgG1
Method of Purification GAPDH antibody was purified by Protein A affinity chromatography.
Form & Buffer Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:1000-100,000

Storage & Safety

Storage Store at 4 deg C for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.

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Images

  • Western Blot analysis using GAPDH antibody (10R-2932 (1)) | 1:2000 (0. 5 ug/ml) antibody dilution used in WB of 5 ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5) and hamster (6).

Availability: In stock

Price: €102.25
Size: 100 ug
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