GAPDH antibody (10R-8399)
Mouse monoclonal GAPDH antibody
Overview
Overview
| Synonyms | Monoclonal GAPDH antibody, Anti-GAPDH antibody, GAPDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, 38 kDa BFA-dependent ADP-ribosylation substrate antibody, aging-associated gene 9 protein antibody, BARS-38 antibody, bb02e05 antibody, BOS_5957 antibody, CDABP0047 antibody, g3pd antibody, G3PDH antibody, gapd antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, glyceraldehyde phosphate dehydrogenase antibody, glyceraldhyde 3-phosphate dehydrogenase antibody, glycerladehyde 3 phosphate dehydrogenase antibody, KNC-NDS6 antibody, Peptidyl-cysteine S-nitrosylase GAPDH antibody |
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| Cross Reactivity | Human |
| Applications | ELISA, IP, WB |
| Immunogen | GAPDH antibody was raised in Mouse using a recombinant human protein purified from E.coli as the immunogen |
Specifications
| Host | Mouse |
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| Clone | 8C2 |
| Isotype | IgG1 kappa |
| Method of Purification | GAPDH antibody was purified by ammonium sulfate precipitation |
| Form & Buffer | Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol |
Usage & Assay Information
| Usage Recommendations | WB: 1:10000, IP: 1-2 ul |
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Storage & Safety
| Storage | Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles |
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General Information
| Biological Significance | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37 kDa subunits. GAPDH catalyzes the reversible reduction of 1,3-bisphos-phoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. |
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