GAPDH antibody (70R-12491)

Affinity purified Rabbit polyclonal GAPDH antibody

Synonyms Polyclonal GAPDH antibody, Anti-GAPDH antibody, GAPD antibody, G3PD antibody, GAPDH antibody, MGC88685 antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, aging-associated gene 9 protein antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody
Cross Reactivity Drosophila, Human, Mouse, Rat
Applications ICC, IF, IHC-P, WB
Immunogen GAPDH antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 335 of GAPDH

Images

Western blot analysis using GAPDH antibody (70R-12491)

Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:10000

Specifications

Host Rabbit
Isotype IgG
Method of Purification GAPDH antibody was purified by antigen-affinity chromatography
Molecular Weight 36 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IHC-P: 1:100-1:1000, IF:1:100-1:500, ICC:1:100-1:500, WB: 1:5000-1:20000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

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Images

  • <div><font face=Calibri size=2>Western blot analysis using GAPDH antibody (70R-12491)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:10000
  • <div><font face=Calibri size=2>Immunohistochemical staining using GAPDH antibody (70R-12491)</font></div>     | Immunohistochemical staining of paraffin-embedded salivary gland cancer tissue using GAPDH antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using GAPDH antibody (70R-12491)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: drosophila) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:5000
  • <div><font face=Calibri size=2>Immunofluorescent staining using GAPDH antibody (70R-12491)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.

Availability: In stock

Price: €302.66
Size: 100 ul
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