Western blot analysis using GAPDH antibody (70R-12491)
Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:10000
GAPDH antibody
Western blot analysis using GAPDH antibody (70R-12491)
Western blot analysis of 30 ug of whole cell lysate (A: Jurkat; B: Raji; C: 293T; D: A431; E: HeLa; F: HepG2; G: H1299; H: HCT116; I: MCF-7; J: NT2D1; K: PC-3; L: U87-MG) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:10000
Immunohistochemical staining using GAPDH antibody (70R-12491)
Immunohistochemical staining of paraffin-embedded salivary gland cancer tissue using GAPDH antibody at a dilution of 1:500
Western blot analysis using GAPDH antibody (70R-12491)
Western blot analysis of 30 ug of whole cell lysate (A: drosophila) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:5000
Immunofluorescent staining using GAPDH antibody (70R-12491)
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:2000.
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
GAPDH antibody was purified by antigen-affinity chromatography
Molecular Weight
36 kDa
Form & Buffer
Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
General Information
Biological Significance
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.