GAPDH antibody (70R-12513)

Affinity purified Rabbit polyclonal GAPDH antibody

Synonyms Polyclonal GAPDH antibody, Anti-GAPDH antibody, G3PD antibody, glyceraldehyde 3-phosphate dehydrogenase antibody, peptidyl-cysteine S-nitrosylase GAPDH antibody, GAPDH antibody, MGC88685 antibody, aging-associated gene 9 protein antibody, GAPD antibody, glyceraldehyde-3-phosphate dehydrogenase antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen GAPDH antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 84 and 175 of GAPDH

Images

Immunofluorescent staining using GAPDH antibody (70R-12513)

Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH antibody at 1:100 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification GAPDH antibody was purified by antigen-affinity chromatography
Molecular Weight 36 kDa
Form & Buffer Supplied as a concentrated soloution containing 1X PBS, 1% BSA, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using GAPDH antibody (70R-12513)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using GAPDH antibody at 1:100 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using GAPDH antibody (70R-12513)</font></div>     | Immunohistochemical staining of paraffin-embedded Gastric CA using GAPDH antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using GAPDH antibody (70R-12513)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 10 % SDS PAGE gel and GAPDH antibody at a dilution of 1:1000

Availability: In stock

Price: €364.83
Size: 100 ul
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