GAPDH antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence at the N-terminal of human GAPDH , identical to the related Rabbit and rat sequences, as the immunogen
Immunocytochemical analysis using using GAPDH antibody (70R-13880)
Immunocytochemistry of MCF1 cells
GAPDH antibody
Immunocytochemical analysis using using GAPDH antibody (70R-13880)
Immunocytochemistry of MCF1 cells
Western Blot analysis using GAPDH antibody (70R-13880)
Lane 1: Rat Cardiac Muscle Tissue Lysate; Lane 2: Rat Brain Tissue Lysate; Lane 3: Rat Liver Tissue Lysate; Lane 4: Rat Skeletal Muscle Tissue Lysate; Lane 5: MM453 Cell Lysate; Lane 6: MM231 Cell Lysate; Lane 7: SMMC Cell Lysate; Lane 8: Hela Cell Lysate; Lane 9: HT1080 Cell Lysate
Specifications
Host
Rabbit
Isotype
IgG
Method of Purification
GAPDH antibody was purified by affinity chromatography
Optimal conditions to be determined by the end user
Storage & Safety
Storage
Store at 4 deg C for short term storage. For long term storage aliquot and store at -20 deg C, avoid repeated freeze/thaw cycles.
General Information
Biological Significance
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.