GBA antibody (70R-12566)

Affinity purified Rabbit polyclonal GBA antibody

Synonyms Polyclonal GBA antibody, Anti-GBA antibody, GBA1 antibody, acid beta-glucosidase antibody, D-glucosyl-N-acylsphingosine glucohydrolase antibody, GCB antibody, GLUC antibody, beta-glucocerebrosidase antibody, imiglucerase antibody, lysosomal glucocerebrosidase antibody, GBA antibody, glucosylceramidase antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen GBA antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 352 and 536 of GBA

Images

Immunofluorescent staining using GBA antibody (70R-12566)

Immunofluorescence analysis of methanol-fixed HeLa, using GBA antibody at 1:100 dilution.

Specifications

Host Rabbit
Isotype IgG
Method of Purification GBA antibody was purified by antigen-affinity chromatography
Molecular Weight 60 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 0.82 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:500, ICC:1:100-1:500, IHC-P: 1:100-1:500, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using GBA antibody (70R-12566)</font></div>     | Immunofluorescence analysis of methanol-fixed HeLa, using GBA antibody at 1:100 dilution.
  • <div><font face=Calibri size=2>Immunohistochemical staining using GBA antibody (70R-12566)</font></div>     | Immunohistochemical staining of paraffin-embedded Lung CA using GBA antibody at a dilution of 1:100
  • <div><font face=Calibri size=2>Western blot analysis using GBA antibody (70R-12566)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A:Hep G2) using a 7.5 % SDS PAGE gel and GBA antibody at a dilution of 1:500

Availability: In stock

Price: €323.11
Size: 100 ul
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