GCSF protein (Mouse) (30R-AG021)
Purified recombinant Mouse GCSF protein
Overview
Overview
| Synonyms | Granulocyte Colony-Stimulating Factor protein, MGI-1G protein, GM-CSFb protein, pluripoietin protein, CSF-3 protein |
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| Species | Mouse |
| Protein Type | Recombinant |
| Applications | User optimized |
Specifications
| Residues | Region of GCSF protein corresponding to amino acids MVPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA. |
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| Expression System | E.coli |
| Grade & Purity | > 98% pure |
| Molecular Weight | 19 kDa |
| Form & Buffer | Lyophilized from 5mM sodium citrate, pH 4.0. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. |
Usage & Assay Information
| Bioactivity | The ED50 was determined by the dose-dependent stimulation of the proliferation of Mouse M-NFS-60 cells is 2 x 10^7 units/mg. |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Endotoxin Levels | Endotoxin level is less than 0.1 ng per ug (1 EU/ug). |
General Information
| Biological Significance | G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and Mouse G-CSF are cross-species reactive. |
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