GDNF protein (30R-2209)
Purified recombinant Human GDNF protein
Overview
Overview
| Synonyms | ATF protein, ATF1 protein |
|---|---|
| Species | Human |
| Protein Type | Recombinant |
| Applications | ELISA, Functional Assay |
| Assay Information | The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 175 pg/ml. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg. |
Specifications
| Expression System | E.coli |
|---|---|
| Grade & Purity | > 98% pure |
| Method of Purification | GDNF protein was purified by affinity chromatography. |
| Molecular Weight | 30 kDa |
| Form & Buffer | Supplied as a sterile liquid with 10 mM phosphate buffer, 150 mMNaCl, 1.0% BSA. 0.22 um filtered. |
Usage & Assay Information
| Bioactivity | The ED50 , calculated by dose-dependent inductionof rat C6 cell proliferation, is 1ug/ml, corresponding to a specific activity of 1x10^3 units/mg. |
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Storage & Safety
| Storage | Store at -70 deg C or less. |
|---|---|
| Endotoxin Levels | Less than 0.01 ng/ug cytokine as determined by the LAL assay. |
General Information
| Biological Significance | Glial Cell derived Neurotrophic Factor, a member of the TGFbeta superfamily, is a neurotrophic factor that promotes the survival of various neuronal populations in both the central and peripheral nervous systems during their development. Neuronal subpopulations affected by GDNF include motor neurons, midbrain dopaminergic neurons and Purkinje cells. GDNF is conserved across mammalian species with human and rat orthologs sharing approximately 93% amino acid sequence identity. E. coli-produced recombinant human GDNF is a disulfide-linked, non-glycosylated homodimer composed of two 135 amino acid polypeptides. |
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