Gelatinase protein (gelE) (30R-3403)
Recombinant Enterococcus faecalis Gelatinase (gelE)
Overview
Overview
| Synonyms | Gelatinase protein, Coccolysin, gelE, Enterococcus faecalis Gelatinase |
|---|---|
| Species | Bacterial |
| Protein Type | Recombinant |
Specifications
| Residues | Amino acids 193-510, full length protein with N-terminal6x His-SUMO-tag. |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 90% pure |
| Molecular Weight | 50.52 kDa |
| Tag/Conjugate | His-Sumo tag |
| Form & Buffer | Lyophilized from Tris-based buffer |
Storage & Safety
| Storage | Store at 4 deg Cshort term, -20 deg Clong term. Avoid repeated freeze thaw cycles. |
|---|
General Information
| Biological Significance | Metalloprotease capable of the hydrolysis of insoluble hydrophobic substrates. Hydrolyzes azocoll and gelatin and, at a lower rate, soluble and insoluble collagens. Does not cleave short synthetic peptides. Preferentially hydrolyzes the 24-Phe-|-Phe-25 bond in the insulin B-chain, followed by the 5-His-|-Leu-6 bond. Inactivates endothelin-1, primarily by cleavage of the 5-Ser-|-Leu-6 and 16-His-|-Leu-17 bonds. Hydrolyzes the alpha chain of C3 to generate a C3b-like protein. Inhibits complement-mediated hemolysis and opsinization of bacteria. Hydrolyzes the insect antimicrobial peptide cecropin. Decreases the length of E.faecalis chains via the activation of autolysin. Degrades polymerized fibrin. |
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| Sequence Information | VGSEVTLK NSFQVAFNVP VEKSNTGIAL HGTDNTGVYH AVVDGKNNYS IIQAPSLVAL NQNAVDAYTH GKFVKTYYED HFQRHSIDDR GMPILSVVDE QHPDAYDNAF WDGKAMRYGE TSTPTGKTYA SSLDVVGHEM THGVTEHTAG LEYLGQSGAL NESYSDLMGY IISGASNPEI GADTQSVDRK TGIRNLQTPS KHGQPETMAQ YDDRARYKGT PYYDQGGVHY NSGIINRIGY TIIQNLGIEK AQTIFYSSLV NYLTPKAQFS DARDAMLAAA KVQYGDEAAS VVSAAFNSAG IGAKEDIQVN QPSESVLVNE |
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