GFP antibody (10R-G103a)

Mouse monoclonal GFP antibody

Synonyms Monoclonal GFP antibody, Anti-GFP antibody, GFP conjugate, Green Fluorescent Protein Antibody
Specificity Jellyfish GFP
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen GFP antibody was raised in mouse using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen.

Images

Flow cytometric assay using GFP antibody (10R-G103a)

GFP antibody is able to detect positive cell population in cytoflow analysis. A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with GFP antibody/fluorescence complex and sorted.

Specifications

Host Mouse
Clone 215
Method of Purification GFP antibody was purified by affinity chromatography.
Form & Buffer 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations ELISA: 1:200,000, IHC: 1:1,000-1:5,000, WB: 1:3,000-1:30,000

Storage & Safety

Storage Store vial at -20 deg C prior to opening. Aliquot and freeze at -20 deg C or below for long term storage. This product is stable for several weeks at 4 deg C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining

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Images

  • Flow cytometric assay using GFP antibody (10R-G103a) | GFP antibody is able to detect positive cell population in cytoflow analysis. A. cells were not stained with anti-GFP antibody/fluorescence complex. B. Cells were stained with GFP antibody/fluorescence complex and sorted.
  • Western blot analysis using GFP antibody (10R-G103a) | Western Blot of GFP antibody. Lane 1: HeLa lysate 50 ng. Lane 2: HeLa lysate 100 ng. Lane 3: HeLa lysate 500 ng. GFP antibody at 1.0 mg/ml for 1 h at room temperature. IRDye800 conjugated Goat-a-Mouse IgG [H&L] at 1:2,500 dilution for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..
  • Western blot analysis using GFP antibody (10R-G103a) | Western Blot of GFP antibody. Lane 1: Molecular Weight Markers. Lane 2: GFP. Load: 10ng per lane. Ms Anti-GFP antibody at 1:1000 for overnight at 4 deg C. Mouse HRP secondary antibody at 1:40,000 for 30 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 27 kDa for epitope tag GFP..
  • Western blot analysis using GFP antibody (10R-G103a) | Western Blot of GFP antibody. Lane 1: 64pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 2: 32pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 3: 16pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 4: 8pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 5: 4pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 6: 2pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 7: 1g of recombinant GFP protein were spiked into a HeLa cell-derived lysates. Lane 8: 0pg of recombinant GFP protein were spiked into a HeLa cell-derived lysates. anti-GFP monoclonal antibody at 1:400 for overnight at 4 deg C. HRP-conjugated anti-Mouse IgG was performed at a dilution of 1:20,000 for 1h at 4 deg C. Block: TTBS supplemented with 1% BSA for 1 h at 4 deg C. Size: 27 kDa for GFP..

Availability: In stock

Price: €399.86
Size: 500 ug
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