GFP antibody (70R-10652)
Affinity purified Chicken polyclonal GFP antibody
Overview
Overview
| Synonyms | Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody |
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| Applications | IF |
| Immunogen | GFP antibody was raised using purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant |
Images
Immunofluorescent staining of GFP antibody (70R-10652)
Dissociated culture of rat cerebral cortical neurons and astrocytes (red staining). The nuclei of some neuronal cells (blue staining) are visible. Picture courtesy of Dr. Gerry Shaw, University of Florida.
Specifications
| Host | Chicken |
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| Method of Purification | GFP antibody was purified by affinity chromatography. |
| Form & Buffer | Supplied in liquid form in 10 mM PBS buffer, pH 7.2, with 0.01% thimerosal and 0.02% sodium azide added as a preservative. |
| Concentration | 10 mg/ml |
Storage & Safety
| Storage | Store at -20 deg C for short term storage. For long term storage, aliquot and store at -80 deg C. Avoid repeated freeze/thaw cycles. Protect from light. |
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General Information
| Biological Significance | Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. |
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