GFP antibody (70R-GG001)
Affinity purified Goat polyclonal GFP antibody
Overview
Overview
| Synonyms | Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody |
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| Specificity | jellyfish GFP |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen. |
Images
Immunofluorescent staining of GFP antibody (70R-GG001)
Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus in Mus musculus (coronal view, 20X magnification). Sf-1:Cre mice were crossed to the Z/EG reporter line. Brains were harvested following cardiac perfusion with 4% PFA/PBS with ON fixation, and subsequently transferred to a 30% sucrose solution. Brains were then frozen in OCT freezing medium and cryostat sectioned at 40 microns. GFP antibody was used at 1:500 dilution in free floating imunnohistochemistry to detect eGFP. Fluorchrome conj ugated anti-Goat IgG secondary antibody was used for detection at 1:500. Sections were counterstained with DAPI.
Specifications
| Host | Goat |
|---|---|
| Method of Purification | GFP antibody was purified by affinity chromatography. |
| Form & Buffer | Sterile filtered liquid in 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3. |
| Concentration | 1.0 mg/ml |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store vial at -20 deg C prior to opening. Aliquot and freeze at -20 deg C or below for long term storage. This product is stable for several weeks at 4 deg C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. |
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