GFP antibody (biotin) (60R-GG001bt)

Goat polyclonal GFP antibody (biotin) conjugated

Synonyms Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein Antibody, GFP conjugate
Specificity Jellyfish GFP
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen GFP antibody (biotin) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen.

Images

Immunofluorescent staining using GFP antibody (biotin) (60R-GG001bt)

issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.

Specifications

Host Goat
Method of Purification GFP antibody (biotin) was purified by affinity chromatography.
Tag/Conjugate Biotin
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

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Images

  • Immunofluorescent staining using GFP antibody (biotin) (60R-GG001bt) | issue: Sf-1:Cre mice crossed to the Z/EG reporter line. Mouse brain (coronal view, 20X magnification). Fixation: 4%PFA/PBS with o/n fixation, and subsequently transferred to a 30% sucrose solution. Antigen retrieval: frozen in OCT freezing medium (Sakura) and cryostat sectioned at 40 microns. Goat anti-GFP was used at 1:500 dilution in free floating imunnohistochemistry to detect GFP. Fluorchrome conjugated Anti-goat IgG secondary antibody was used for detection at 1:500 at 1:10,000 for 45 min at RT. Localization: Sf-1+ neurons and their processes of the ventromedial nucleus of the hypothalamus. Staining: eGFP as green fluorescent signal and sections were counterstained with DAPI.
  • Immunofluorescent staining using GFP antibody (biotin) (60R-GG001bt) | Tissue: Drosophila melanogaster late stage embryonic central nervous system. Fixation: 0.5% PFA. Antigen retrieval: not required. Anti-GFP antibody at a 1:1,000 for 1 h at RT. AlexaFluor 488 conjugated anti-Goat antibody at 1:300 for 45 min at RT. Panel A: shows a lateral view (ventral left). Panels B and C: shows ventral views of whole mount embryos at 63x magnification (plus 2x digital zoom). In all panels, anterior is up. Staining: tau-GFP cell bodies (large arrowhead) and axons of motorneurons (arrow) and interneurons (small arrowhead) as green fluorescent signal.
  • Western blot analysis using GFP antibody (biotin) (60R-GG001bt) | Western blot of Biotin GFP antibody. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for GFP..
  • Western blot analysis using GFP antibody (biotin) (60R-GG001bt) | Western Blot of GFP antibody. Lane 1: HeLa cells. Lane 2: mock transfected HeLa cell lysate. Load: 35 ug per lane. GFP antibody at 1 ug/ml for 1 h at room temperature. IRDye® 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (605-732-125) secondary antibody at 1:2,500 for 45 min at RT. Block: 5% Blocking buffer overnight at 4 deg C. Size: 27 kDa, 33 kDa for GFP..

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Price: €597.44
Size: 1 mg
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