GFP antibody (biotin) (60R-GG001bt)
Goat polyclonal GFP antibody (biotin) conjugated
Overview
Overview
| Synonyms | Polyclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein Antibody, GFP conjugate |
|---|---|
| Specificity | Jellyfish GFP |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | GFP antibody (biotin) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria as the immunogen. |
Images
Western blot analysis using GFP antibody (biotin) (60R-GG001bt)
Western blot of Biotin GFP antibody. Lane 1: GFP. Lane 2: None. Load: 50 ng per lane. GFP antibody Biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 28 kDa, 28 kDa for GFP..
Specifications
| Host | Goat |
|---|---|
| Method of Purification | GFP antibody (biotin) was purified by affinity chromatography. |
| Tag/Conjugate | Biotin |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000 |
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Storage & Safety
| Storage | Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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| Biohazard Information | This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product. |
General Information
| Biological Significance | Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. |
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