GFP antibody (HRP) (61R-G103aHRP)
Mouse monoclonal GFP antibody (HRP)
Overview
Overview
| Synonyms | Monoclonal GFP antibody, Anti-GFP antibody, GFP conjugate, Green Fluorescent Protein Antibody |
|---|---|
| Specificity | Jellyfish GFP |
| Cross Reactivity | To be determined by end-user |
| Applications | ELISA, IHC, WB |
| Immunogen | GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. as the immunogen. |
Images
Western blot analysis using GFP antibody (HRP) (61R-G103aHRP)
Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. Anti-GFP antibody HRP secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP..
Specifications
| Host | Mouse |
|---|---|
| Clone | 215 |
| Method of Purification | GFP antibody (HRP) was purified by affinity chromatography. |
| Tag/Conjugate | HRP |
| Form & Buffer | Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% thimerisol. Immunoglobulin and protease free. |
Usage & Assay Information
| Usage Recommendations | ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000 |
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Storage & Safety
| Storage | Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use. |
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General Information
| Biological Significance | Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. |
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