GFP antibody (HRP) (61R-G103aHRP)

Mouse monoclonal GFP antibody (HRP)

Synonyms Monoclonal GFP antibody, Anti-GFP antibody, GFP conjugate, Green Fluorescent Protein Antibody
Specificity Jellyfish GFP
Cross Reactivity To be determined by end-user
Applications ELISA, IHC, WB
Immunogen GFP antibody (HRP) was raised in Mouse using The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. as the immunogen.

Images

Western blot analysis using GFP antibody (HRP) (61R-G103aHRP)

Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. Anti-GFP antibody HRP secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP..

Specifications

Host Mouse
Clone 215
Method of Purification GFP antibody (HRP) was purified by affinity chromatography.
Tag/Conjugate HRP
Form & Buffer Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% thimerisol. Immunoglobulin and protease free.

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000

Storage & Safety

Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.

General Information

Biological Significance Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

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Images

  • Western blot analysis using GFP antibody (HRP) (61R-G103aHRP) | Western Blot of GFP antibody (HRP). Lane 1: 50ng of GFP. Lane 2: none. none. Anti-GFP antibody HRP secondary antibody was used at 1:1000 for 45 min at RT. Block: 1% BSA-TTBS (diluted to 1X) 30 min at 20 deg C. Size: 28 kDa for GFP..

Availability: In stock

Price: €842.06
Size: 1 mg
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