Ghrelin protein (His tag) (80R-1210)
Purified recombinant Human Ghrelin protein
|Synonyms||Ghrelin 28 protein, Growth hormone releasing peptide protein, Ghrelin-28 protein, Ghrelin 27 protein, Ghrelin/obestatin prepropeptide protein, MTLRP protein, M46 protein protein, Motilin related peptide protein, , M-46 protein protein, Obestatin protein, Growth hormone secretagogue protein, Ghrelin-27 protein, GHRL protein, Appetite-regulating hormone Appetite regulating hormone protein|
Coomassie Blue stained SDS-PAGE of Ghrelin protein (His tag) (80R-1210)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||24-117 amino acids: MGSSHHHHHH SSGLVPRGSH MGSSFLSPEH QRVQQRKESK KPPAKLQPRA LAGWLRPEDG GQAEGAEDEM EVRFNAPFDV GIKLSGVQYQ QHSQALGKFL QDILWEEAKE APADK|
|Grade & Purity||> 90% pure|
|Molecular Weight||12.8 kDa (115aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method )|
|Biological Significance||Ghrelin, also known as Obestatin, is the ligand for growth hormone secretagogue receptor type 1 (GHSR). It induces the release of growth hormone from the pituitary and involved in growth regulation. This protein has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Ghrelin plays a significant role in neurotrophy, particularly in the hippocampus, and is essential for cognitive adaptation to changing environments and the process of learning. Recombinant Ghrelin protein was expressed in E. coli and purified by using conventional chromatography techniques.|
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