GLDA protein (80R-4268)
Recombinant E.coli GLDA protein with His tag
Overview
Overview
| Synonyms | E.coli GLDA protein, Recombinant GLDA protein, Glycerol dehydrogenase protein, ECK3937 protein, JW5556 protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | Control, SDS-PAGE |
| Assay Information | (1) Prepare a 200 ul reaction mix with final concentrations of 93mM Glycine, 93mM Potassium chloride, 2375mM Glycerol, 3mM b-NAD. (2) Equilibrate to 25 deg C and monitor at A340nm until the reading is constant using a spectrophotometer. (3) Add 20 ul of recombinant GLDA protein at different concentrations (0.2 ug, 0.1 ug, 0.05 ug) in 180 ul of the prepared reaction buffer. (4) Invert to mix and read the decrease at A340nm for 10 minutes. |
Images
Coomassie blue stained SDS-PAGE showing analysis of GLDA protein(80R-4268)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-367 amino acids of E.coli GLDA protein: MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 41.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in PBS (pH 7.4) containing 10% glycerol |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | One unit will oxidize 1.0 umole of glycerol to dihydroxyacetone per minute at pH 8.0 at 25 deg C. Specific activity: > 14 units/ml. |
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Storage & Safety
| Storage | Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles. |
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General Information
| Biological Significance | gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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