gldA protein (His tag) (80R-3959)

Recombinant E.coli gldA protein

Synonyms Recombinant gldA protein Glycerol dehydrogenase protein, ECK3937 protein, JW5556 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie blue stained SDS-PAGE showing analysis of gldA protein (His tag) (80R-3959)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-367 aa; MGSSHHHHHH SSGLVPRGSH MGSMDRIIQS PGKYIQGADV INRLGEYLKP LAERWLVVGD KFVLGFAQST VEKSFKDAGL VVEIAPFGGE CSQNEIDRLR GIAETAQCGA ILGIGGGKTL DTAKALAHFM GVPVAIAPTI ASTDAPCSAL SVIYTDEGEF DRYLLLPNNP NMVIVDTKIV AGAPARLLAA GIGDALATWF EARACSRSGA TTMAGGKCTQ AALALAELCY NTLLEEGEKA MLAAEQHVVT PALERVIEAN TYLSGVGFES GGLAAAHAVH NGLTAIPDAH HYYHGEKVAF GTLTQLVLEN APVEEIETVA ALSHAVGLPI TLAQLDIKED VPAKMRIVAE AACAEGETIH NMPGGATPDQ VYAALLVADQ YGQRFLQEWE
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 41.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as liquid in PBS (pH 7.4), 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for 1-2 weeks. For long term storage, aliquot and store at -20 to -70 deg C. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance gldA catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). This protein allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. In E.coli, an important role of GldA is also likely to regulate the intracellular level of dihydroxyacetone by catalyzing the reverse reaction, i.e. the conversion of dihydroxyacetone into glycerol. gldA possesses a broad substrate specificity, since it is also able to oxidize 1,2-propanediol and to reduce glycolaldehyde, methylglyoxal and hydroxyacetone into ethylene glycol, lactaldehyde and 1,2-propanediol, respectively. Recombinant E. coli gldA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • 

Coomassie blue stained SDS-PAGE showing analysis of gldA protein (His tag) (80R-3959)



 | 

Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €300.20
Size: 100 ug
OR
Shipping
View Our Distributors