GLE1 antibody (70R-13059)

Affinity purified Rabbit polyclonal GLE1 antibody

Synonyms Polyclonal GLE1 antibody, Anti-GLE1 antibody, nucleoporin GLE1 antibody, LCCS1 antibody, GLE1L antibody, GLE1 antibody, hGLE1 antibody, GLE1 RNA export mediator homolog (yeast) antibody, GLE1-like RNA export mediator antibody, GLE-1 antibody, GLE 1, GLE-1, GLE 1 antibody, GLE1, LCCS antibody, GLE1-like protein antibody
Cross Reactivity Human
Applications ICC, IF, IHC-P, WB
Immunogen GLE1 antibody was raised in rabbit using a recombinant protein fragment contain a sequence corresponding to a region within amino acids 414 and 646 of GLE1

Images

Immunofluorescent staining using GLE1 antibody (70R-13059)

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GLE1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.

Specifications

Host Rabbit
Isotype IgG
Method of Purification GLE1 antibody was purified by antigen-affinity chromatography
Molecular Weight 75 kDa
Form & Buffer Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF:1:100-1:1000, ICC:1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.

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Images

  • <div><font face=Calibri size=2>Immunofluorescent staining using GLE1 antibody (70R-13059)</font></div>     | Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GLE1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
  • <div><font face=Calibri size=2>Immunohistochemical staining using GLE1 antibody (70R-13059)</font></div>     | Immunohistochemical staining of paraffin-embedded Cal27 xenograft using GLE1 antibody at a dilution of 1:500
  • <div><font face=Calibri size=2>Western blot analysis using GLE1 antibody (70R-13059)</font></div>     | Western blot analysis of 30 ug of whole cell lysate (A: HeLa) using a 7.5 % SDS PAGE gel and GLE1 antibody at a dilution of 1:1000

Availability: In stock

Price: €323.11
Size: 100 ul
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