GLO1 protein (80R-4289)

Purified Recombinant GLO1 protein (His tagged)

Synonyms Glyoxalase I protein, Glx 1 protein, GLO-1 Methylglyoxalase protein, Aldoketomutase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using GLO1 protein (His tag) (80R-4289)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-184 aa: ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
Expression System E.coli
Grade & Purity > 90% pure
Molecular Weight 20.7 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity > 400 units/mg

Storage & Safety

Storage Store at 4 deg C for short term storage. For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeat freeze/thaw cycles
Endotoxin Levels < 1.0 EU per 1 microgram of protein

General Information

Biological Significance Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

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SDS-PAGE analysis using GLO1 protein (His tag) (80R-4289)



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Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €102.25
Size: 20 ug
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