glpE protein (His tag) (80R-2849)

Purified recombinant glpE protein (His tag)

Synonyms glpE, Thiosulfate:cyanide sulfurtransferase (rhodanese) protein, ECK3411 protein, JW3388 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of glpE protein (His tag) (80R-2849)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-108 aa: MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 14.5 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Thiosulfate:cyanide sulfurtransferase (rhodanese), also known as glpE, is a mitochondrial matrix enzyme that is encoded by the nucleus. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. Recombinant E.coli glpE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • SDS-PAGE analysis of glpE protein (His tag) (80R-2849) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €224.95
Size: 100 ug
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