GLPK protein (80R-4200)
Recombinant E.coli GLPK protein with His tag
Overview
Overview
| Synonyms | E.coli GLPK protein, Recombinant GLPK protein, Glycerol kinase protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | Control, SDS-PAGE |
Images
Coomassie blue stained SDS-PAGE showing analysis of GLPK protein(80R-4200)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-847 amino acids of E.coli GLPK protein: MGSSHHHHHH SSGLVPRGSH MGSMTEKKYI VALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAA IGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILD HVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPE VRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTG EVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFG LTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVR EVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 58.6 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as liquid in PBS (pH 7.4) containing 10% glycerol and 1mM DTT |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles. |
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General Information
| Biological Significance | glpK also known as glycerol kinase, belongs to the FGGY kinase family. glpK function to catalyze the transfer of a phosphate group from ATP to glycerol, thereby forming glycerol phosphate. This intermediate can then be converted to dihydroxyacetone phosphate (DHAP), which is utilized in either glycolysis or gluconeogenesis. Recombinant E.coli glpK, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
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