GLPK protein (80R-4200)

Recombinant E.coli GLPK protein with His tag

Synonyms E.coli GLPK protein, Recombinant GLPK protein, Glycerol kinase protein
Species E.coli
Protein Type Recombinant
Applications Control, SDS-PAGE

Images

Coomassie blue stained SDS-PAGE showing analysis of GLPK protein(80R-4200)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-847 amino acids of E.coli GLPK protein: MGSSHHHHHH SSGLVPRGSH MGSMTEKKYI VALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAA IGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILD HVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPE VRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTG EVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFG LTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVR EVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 58.6 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as liquid in PBS (pH 7.4) containing 10% glycerol and 1mM DTT
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles.

General Information

Biological Significance glpK also known as glycerol kinase, belongs to the FGGY kinase family. glpK function to catalyze the transfer of a phosphate group from ATP to glycerol, thereby forming glycerol phosphate. This intermediate can then be converted to dihydroxyacetone phosphate (DHAP), which is utilized in either glycolysis or gluconeogenesis. Recombinant E.coli glpK, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • 

Coomassie blue stained SDS-PAGE showing analysis of GLPK protein(80R-4200)



 | 

Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: €298.57
Size: 100 ul
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