glsA1 protein (His tag) (80R-1810)
Purified recombinant E.coli glsA1 protein
Overview
Overview
| Synonyms | glsA-1 protein, ybaS protein, glsA1, glsA-1, glsA 1 protein, glsA 1, Glutaminase1 protein |
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| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Images
Coomassie Blue stained SDS-PAGE of glsA1 protein (His tag) (80R-1810)
Figure annotation denotes ug of protein loaded and % gel used.
Specifications
| Residues | 1-310 amino acids: MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG |
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| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 35.0 kDa (330aa), confirmed by MALDI-TOF |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 100mM NaCl. |
| Concentration | 1 mg/ml |
Storage & Safety
| Storage | Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
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General Information
| Biological Significance | GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+. Recombinant E. coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
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