Glucokinase protein (80R-4380)
Purified Recombinant Glucokinase protein (His tagged)
Overview
Overview
| Synonyms | glk protein, ECK2384 protein, JW2385 protein |
|---|---|
| Species | E.coli |
| Protein Type | Recombinant |
| Applications | SDS-PAGE |
Specifications
| Residues | 1-321 aa: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL |
|---|---|
| Expression System | E.coli |
| Grade & Purity | > 95% pure |
| Molecular Weight | 37.1 kDa |
| Tag/Conjugate | His tag |
| Form & Buffer | Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol. |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Bioactivity | Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of B |
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Storage & Safety
| Storage | Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles. |
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General Information
| Biological Significance | glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. |
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