Glucokinase protein (80R-4380)

Purified Recombinant Glucokinase protein (His tagged)

Synonyms glk protein, ECK2384 protein, JW2385 protein
Species E.coli
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis using Glucokinase protein (His tag) (80R-4380)

Specifications

Residues 1-321 aa: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
Expression System E.coli
Grade & Purity > 95% pure
Molecular Weight 37.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of B

Storage & Safety

Storage Store at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate.

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Images

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SDS-PAGE analysis using Glucokinase protein (His tag) (80R-4380)



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Availability: In stock

Price: €126.79
Size: 20 ug
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